IP Archives of Cytology and Histopathology Research

Print ISSN: 2581-5725

Online ISSN: 2456-9267


IP Archives of Cytology and Histopathology Research (ACHR) open access, peer-reviewed quarterly journal publishing since 2016 and is published under the Khyati Education and Research Foundation (KERF), is registered as a non-profit society (under the society registration act, 1860), Government of India with the vision of various accredited vocational courses in healthcare, education, paramedical, yoga, publication, teaching and research activity, with the aim of faster and better dissemination more...

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Prabhu P, Sandhya I, Rao, and Alwa: A descriptive study of Endometrial Biopsies in perimenopausal women having Abnormal uterine bleeding


Abnormal uterine bleeding (AUB) is one of the commonest symptom with which the perimenopausal female visits the gynaecologist accounting for over 70% of all gynecological consultations in that age group.1 The clinical presentation of AUB can range anywhere menorrhagia, polymenorrhea, polymenorrhagia, metrorrhagia, and menometrorrhagia.2

Studies done over the past few years have shown that in the past few decades there is a rising trend increase in the incidence of endometrial adenocarcinoma, especially in the Indian subcontinent.3

Though biopsy is the gold standard it is an invasive procedure and hence often an Ultrasound is used to evaluate the pathology of the endometrium routinely. Hence, we decided to study the accuracy between radiological ET thickened and its usefulness in detecting endometrial pathology.

Materials and Methods

This study was carried out in the Department of Pathology at AJIMS, Mangalore for a period of 2 years. The study was started after obtaining ethical clearance from the institutions ethical clearance committee.

Patients between the age of 40 and 55 who were confirmed by history to be in the perimenopausal period were selected and explained of the study. Those who gave informed written consent to participate in the study were chosen. These patients underwent ultrasound in which the endometrial thickness was measured at 3 levels and an average noted, the endometrial volume was also noted, following this a pre anaesthetic check-up was done and the patient was posted for biopsy, in those who underwent endometrial biopsies at least 4 samples of tissue was collected for biopsy. Those who had adequate samples were selected sampled and the histology was co related with the ET. A cut off of 4 mm was taken to differentiate between benign and malignant disease.

The sample included endometrial biopsies and hysterectomy specimens of perimenopausal women for AUB. The ultrasonographic findings and Histopathological (HPE) reports were analysed.


Among 101 cases for AUB, 67 cases were diagnosed as non secretory endometrium. Out of these 67 cases, 49 cases were associated with fibroids. Out of the rest of the AUB cases, 31 of them were diagnosed to have endometrial hyperplasia and 3 cases were diagnosed to have endometrial carcinoma. USG thickness of 17(54%) out of 31 endometrial hyperplasia cases were increased.

The age group in the study ranged between 40 and 50 years the mean age was 47.89 SD + 5.91 years.

These were our findings

Figure 1
Table 1

Type of endometrium



non secretory endometrium



Endometrial lesions



non secretory endometrium






No fibroids





endometrial hyperplasia



endometrial carcinoma



Endometrial hyperplasia(31)



Increased USG thickness



USG thickness normal



Table 2



95% CI



44.68% to 84.37%



42.13% to 99.64%

Positive Likelihood Ratio


0.74 to 29.28

Negative Likelihood Ratio


0.20 to 0.74

Disease prevalence (*)


58.90% to 90.41%

Positive Predictive Value (*)


71.83% to 99.01%

Negative Predictive Value (*)


28.31% to 58.76%

Accuracy (*)


51.96% to 85.78%


After ruling out medical reasons, endometrial biopsy or curettage might be a safe and useful diagnostic step in the examination of AUB. The perimenopausal age group is the commonest age group affected in endometrial pathologies shows studies done by Soleymani E et al.4 Shwetha Agrawal et al.5 Jagadale Kunda et al.6 Out of 101 AUB cases, 3 cases were endometrial malignancy a similar study done by K. Gruboeck et al7 noted that 11.34% of cases had evidence of endometrial malignancy and in them the mean ET was 29.5 mm + SD 12.59 and the mean volume was 39.0 ml + SD 34.16). 88.66% had endometrial hyperplasia and 7.22 had endometrial polyps and in them the mean ET volume was 15.64 mm (SD 5.26) and 5.47 ml (SD 6.32), respectively.

Richard J. Persadi et al8 did a met analytical study and stated that the use of TVS has a good sensitivity to find out endometrial cancer and whenever the thickness is more than 5 mm it needs to be sampled to rule of the possibility of malignant disease. In our study 17 out of 31 cases of endometrial hyperplasia showed increased endometrial thickness. Remaining 14 showed normal endometrial thickness but with endometrial hyperplasia. A study done by Janesh K Gupta et al9 in their meta analysis noted that ultrasound size of ET only, cannot be used to precisely rule out pathology . However, a negative result at < or = 5 mm cut-off level measuring both endometrial layers in the presence of endometrial pathology rules out endometrial pathology with good certainty. Sreelakshmy Krishnankutty Nair et al10 at a cut off of 4mm noted that transvaginal sonography showed a sensitivity of 71.79%, specificity of 100%, positive predictive value of 100% and negative predictive value of 50% overall.

The sensitivity of transvaginal sonography in detecting premalignant lesions was 75.76%, specificity was 82.35%, positive predictive value was 89.29% and negative predictive value was 63.64%. Ritu Mishra et al11 study showed the sensitivity, PPV and specificity of TVS were 73.9%, 77.3% and 73.7%. Cacciatore et al12 For TVS the sensitivity and specificity versus endometrial pathology were 73.9% and 73.7%.


Radiological and pathological evaluation were concordant with diagnosis of AUB associated with fibroids. However only 54% of the endometrial hyperplasia were found to have abnormal endometrial thickness. Radiological evaluation of endometrial thickness is not concordant in 46% of the cases. This study emphasizes on histopathological examination in perimenopause women with abnormal uterine bleeding with normal endometrial thickness on sonographic findings.

Conflict of Interest

The authors declare that there are no conflicts of interest in this paper.

Source of Funding




N Mahajan M Aggarwal A Bagga Health issues of menopausal women in North IndiaJ Midlife Health201232847


P Kumar N Malhotra P Kumar Clinical types of abnormal uterine bleedingJeffcoate’s Principle of Gynecology. 7th Edn.Jaypee Brothers Medical PublishersNew Delhi2008599


S C Faria T Sagebiel A Balachandran Imaging in endometrial carcinomaIndian J Radiol Imaging201525213747


E Soleymani K Ziari O Rahmani Histopathological findings of endometrial specimens in abnormal uterine bleedingArch Gynecol Obstet201428948459


S Agrawal A Mathur K Vaishnav Histopathological study of endometrium in abnormal uterine bleeding in women of all age groups in western rajasthan (400 cases)Int J Basic Appl Med Sci201443158


J Kunda S Anupam Histopathological study of endometrium in abnormal uterine bleeding in reference to different age groups, parity and clinical symptomatologyInt J Clin and Biomed Res201512905


K Gruboeck D Jurkovic F Lawton M Savvas A Tailor S Campbell The diagnostic value of endometrial thickness and volume measurements by three-dimensional ultrasound in patients with postmenopausal bleedingUltrasound Obstet Gynecol1996842726


R J Persadie Ultrasonographic assessment of endometrial thickness: a reviewJ Obstet Gynaecol Canada20022421316


J K Gupta P F Chien D Voit T J Clark K S Khan Ultrasonographic endometrial thickness for diagnosing endometrial pathology in women with postmenopausal bleeding: a meta-analysisActa Obstet Gynecol Scand200281979981610.1034/j.1600-0412.2001.810902.x


S K Nair R V Nair A Rajendran Comparative study of transvaginal ultrasound with histopathology in diagnosing endometrial pathology in postmenopausal bleedingJ Evol Med Dent Sci20165146402


R Mishra AP Misra Y Mangal To Compare the Results of TVS and SIS with Hysteroscopy and Histopathological Examination in Perimenopausal & Postmenopausal Bleeding”J Evol Med Dent Sci20154712307


B Cacciatore T Ramsoy P Lehtoirta P Ylostalo Transvaginal sonography and hysteroscopy in postmenopausal bleedingActa Obstet Gynecol Scand19947354136


© This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Authors Details

Manjushree Prabhu P, Sandhya I, Purnima S Rao, Sameeksha Alwa

Article History

Received : 15-07-2021

Accepted : 23-08-2021

Available online : 16-09-2021

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